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Plasmodium falciparum chitinase (PfCHT1) with 3 cysteines had been expressed from a plasmid under the regulation of T7 promoter.
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Right after induction, cells have been harvested and crude mobile lysates have been geared up. PfCHT1 was assayed using a chromogenic substrate (CalBioChem #474550) and standardized to protein focus using Bradford reagent.
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